Product category:
Polymerase chain reaction (PCR)
News Release from: Thermo Fisher Scientific | Subject: ReddyMix PCR Buffer
Edited by the Laboratorytalk Editorial
Team on 15 May 2007
PCR buffer eliminates post-amplification
steps
Thermo has developed a PCR buffer that enables samples to be loaded directly onto an agarose gel after thermal cycling, reducing the number of pipetting and reagent handling steps in PCR protocols
The Thermo Scientific ReddyMix PCR Buffer is specially formulated with a red tracker dye and precipitant in the reaction buffer Utilisation of mixes containing this buffer allows laboratories to avoid having to buy and utilise separate loading dyes when loading PCR products on to agarose gels for electrophoresis and subsequent visualisation, thus saving both time and money
This article was originally published on Laboratorytalk on 16 Jul 2008 at 8.00am (UK)
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The red dye in ReddyMix migrates between bromophenol blue and xylene cyanol at approximately 300bp, depending on the agarose concentration.
Since the additives are contained in the reaction buffer rather than in the enzyme preparation, the red dye and precipitant are present at optimal concentrations in the final mix.
This contrasts favourably with products that contain dye and precipitant in the enzyme formulation, as these tend to sink slowly and erratically into test plate wells, producing dye fronts that are hard to see.
By reducing pipetting and reagent handling steps in the PCR process, use of ReddyMix reduces contamination risks and enhances testing reproducibility.
This means that standard tests can be set up for purposes such as screening or teaching, enabling users to be confident that results will be consistent every time.
ReddyMix has no adverse effect on the outcome of PCR, and yields are exactly the same as would be achieved with standard polymerase products.
Use of the product does not interfere with downstream applications.
ReddyMix is available across the whole of Thermo Scientific's PCR reagent range.
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