Cell viability analysis made more reliable
An
Agilent Automation (formerly Velocity11) product story
Edited by the Laboratorytalk editorial team
Nov 7, 2007
Velocity11 has released a new application brief describing an automated cell viability workstation based on its Bravo liquid handler, BenchCel plate handling system, and CellTiter-Glo kit from Promega
The automated cell viability workstation is shown to provide a reliable, efficient solution for analysing cell viability, says Velocity11.
Using the described protocol up to 65 plates can be run without user intervention.
The typical throughput for the described set-up was found to be about three hours for 50 plates, depending on exact protocol and liquid handling steps.
Cell viability testing is an important part of the Adme-Tox compound profiling approach.
The demand for these type of assays is high due to the increasing output from high throughput screening (HTS) and the pressure to front-load as much toxicity testing as possible during the drug discovery process.
There are a number of different approaches for cell viability testing.
In this new application brief the authors outline the protocol using the Promega CellTiter-Glo kit as it is ideally suited for adaptation on automated workstations.
The combination of Velocity11's Bravo liquid handler and the BenchCel plate handling system is shown to allow users to run the assay with high throughput and maximum walk-away time.
Plates are delivered to the Bravo, reagent is added and plates are placed on shaking devices.
The reagent is added without tip touching, therefore only one tip-box is needed for the total run.
The plates are transported back to the BenchCel for room temperature incubation, directly followed by signal detection on a microplate reader.
The automated workstation ensures that all process and incubation times are accurately managed, to ensure reliable and repeatable results.
Not what you're looking for? Search the site.
Google Ads
Contact Agilent Automation (formerly Velocity11)
Related Stories
- Mettler Toledo re-launches balance portfolio
- Microfluidic device enables high-throughput mixing
- Distillation system produces ultra-pure acids
- Milestone releases updated Ultraclave IV
- Submersible magnetic stirrers start up jerk-free
Articles by product category
-
Analytical instruments (8,805)
- Analytical instruments (13)
- Chemical analysis equipment (631)
- Chromatographs: gas (241)
- Chromatographs: liquid, ion, gel, HPLC (427)
- Chromatography accessories (503)
- Gas analysis equipment (219)
- pH and conductivity meters (114)
- Cameras and imaging systems (820)
- Electron microscopy systems (209)
- Optical microscopes (448)
- Microscope supplies and accessories (558)
- Mass spectrometers (458)
- Spectroscopy (535)
- Other analytical instruments (592)
- Particle size analysis equipment (555)
- Microplate readers (39)
- Temperature, pressure and flow measurement (262)
- Spectrophotometers (273)
- Titration equipment (655)
- Ultrasound equipment and ultrasonic spectroscopy (118)
- Viscometers and rheometers (346)
- Oscilloscopes (83)
- Mechanical testing equipment (492)
- Test and calibration instrumentation (214)
-
Life sciences and clinical laboratory equipment (3,579)
- Life sciences and clinical laboratory equipment (3)
- Clinical chemistry analysis (898)
- Nucleic acid sequencing and synthesis (637)
- Genomics (459)
- Proteomics (450)
- Assay kits (185)
- Microbiology (383)
- Polymerase chain reaction (PCR) (240)
- Cell/tissue handling and counting (324)
-
Computer hardware (826)
- Computer hardware (7)
- Dataloggers (302)
- I/O devices (362)
- Monitors and peripherals (50)
- Process control and networking (79)
- PLCs (26)
-
Industry news (19)
- Industry news (19)
-
Laboratory equipment and wares (5,937)
- Laboratory equipment and wares (5)
- Safety and ventilation equipment (322)
- General lab equipment (831)
- Workstations (61)
- Detectors, sensors and probes (375)
- Lab automation equipment and robotics (651)
- Laboratory furniture and storage (151)
- Gas handling (125)
- Heating: ovens, furnaces, autoclaves (331)
- Fermentation and incubation equipment (73)
- Microplates/microarrays and readers (397)
- Microfluidics/Lab-on-a-chip systems (142)
- Distillation equipment (16)
- Sampling equipment (156)
- Environmental chambers (88)
- Fume cupboards ductless (79)
- Laboratory glassware (400)
- Liquid handling (415)
- Cooling: refrigeration (198)
- Washing and sterilisation equipment (207)
- Fume cupboards ducted (31)
- HTS and combinatorial chemistry equipment (57)
- Evaporation equipment (166)
- Weighing and balancing (240)
- Centrifuges (47)
- Water purification (213)
- Laboratory plasticware (160)
-
Laboratory supplies and consumables (1,271)
- Laboratory supplies and consumables (2)
- Culture media (119)
- Reagents and catalysts (169)
- Antibodies (203)
- Cleaning products (65)
- Reference standards (178)
- Chemicals and biochemicals (534)
-
Pumps, Valves, Filters (708)
- Automated Valves (66)
- Filtration equipment and supplies (176)
- Metering pumps (109)
- Other pumps (63)
- Peristaltic pumps (65)
- Vacuum pumps and compressors (144)
- Manual Valves (85)
-
Services (2,989)
- Contract research (889)
- Laboratory and scientific exhibitions and conferences (230)
- Laboratory and scientific recruitment (26)
- Laboratory and scientific consultancy services (288)
- Laboratory and scientific training and education (413)
- Laboratory building and design (50)
- Laboratory and scientific manufacturing services (136)
- Laboratory and scientific professional organisations (482)
- Laboratory and scientific books and publishing (475)
-
Computer software (2,882)
- Computer software (4)
- Chemical modelling software (181)
- Laboratory and scientific databases (327)
- Instrument control software (320)
- Laboratory information management system (LIMS) (721)
- Mathematical software (163)
- Process control and networking software (52)
- Data acquisition software (171)
- Chromatography software (284)
- Spectroscopy software (92)
- Analytical, statistical and report generating software (284)
- Data management software (283)
