Bio-photometric detection using dual beams
LEDspec is a stand alone LED-based bio-photometric detection system targeted at flow analysis and single-scan applications
LEDspec can be equipped with up to seven LEDs of different wavelength.
Its noise (< 0.1mAU peak to peak) and drift performance (<0.5mAU/h) exceeds a photodiode array based detection system at a fraction of the cost.
LEDspec is based on a dual-beam approach.
Light sources related baseline drift caused by warm up, temperature stability and bulb aging in conventional single beam spectrometer systems is avoided.
A reference channel in the LEDspec corrects for baseline simultaneously with the sample measurement.
Life time of the LED modules is virtually unlimited.
LEDspec allows measurement of absorbance, transmission or intensity at a single wavelength or at multiple wavelengths.
When the detector is setup for single wavelength detection, very precise fluid injection analysis systems can be assembled.
Using two wavelengths, baseline offsets caused by, for instance, particles in the sample stream can be observed and automatically subtracted.
Measurements at several wavelengths allow for multi-wavelength calibration.
The result of the measurement can be calculated, calibrated and scaled to the user's desirable dynamic range.
LEDspec is ideally suited for WPI's fibre optic sampling equipment.
High sensitivity detection systems for flow analysis can be assembled using WPI's liquid waveguide capillary cells (LWCC) with effective pathlengths ranging from 2 to 500cm.
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