Product category:
Chromatography accessories
News Release from: Waters | Subject: XBridge
Edited by the Laboratorytalk Editorial
Team on 05 July 2005
Columns set chromatography benchmark
Hybrid particles combine the efficiencies of silica-based materials with the pH resistance more common to polymer packing materials giving a leap in high pH phase stability
Method development scientists looking to maximise efficiency and further improve pH stability and column reliability for reverse phase chromatography should look no further, says Waters The company announced the launch of the XBridge HPLC column family at the International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2005)in Stockholm, Sweden
This article was originally published on Laboratorytalk on 26 Feb 2007 at 8.00am (UK)
Related stories
Oligonucleotide separations technology from Waters
Waters has announced the introduction of two new sets of columns for UPLC and HPLC for the purification and analysis of oligonucleotides
Enabling technology for E-lab notebook
Diosynth selects Waters NuGenesis Scientific data management solution for its internal E-lab notebook initiative
The XBridge column family is a major expansion of Waters second generation ethylene-bridged hybrid particle line (BEH technology).
These hybrid particles combine the efficiencies of silica-based materials with the pH resistance more common to polymer packing materials.
BEH technology particles were first introduced as an enabling technology for the Acquity UPLC systems at the Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy in 2004, and expanded upon in 2005.
Further reading
Apotex selects scientific data management system
Company plans to reduce cycle time in new product development by standardising on data management platform, which will be deployed at up to 15 sites worldwide
Taking liquid chromatography a stage further
Claiming to be the first end-to-end ultra performance liquid chromatography system offering specially designed column chemistries, hardware, software, and documentation
Expanded chromatography control with this software
Acquires data and controls the operation of and processes data from Waters brand instrumentation as well as Agilent photodiode array detectors, liquid chromatographs, and gas chromatographs
When used in a UPLC system, these 1.7um BEH particles are characterised by their superior attributes of mechanical strength, pH resistance, and ultra high efficiencies, says the company.
This announcement expands BEH technology into HPLC and preparative chromatography markets with the release of the larger XBridge HPLC column particle sizes.
Waters says this launch demonstrates its commitment to continued investment in particle research and development to provide reliable solutions for complex method development problems.
According to Damian Morrison, column technology manager, "XBridge's significant leap in high pH phase stability will change the landscape of chromatography by allowing method developers to perform separations of the highest power.
"They can be confident that even the most challenging of sample mixtures can be separated more efficiently, significantly reducing method development time and complexity".
Based upon detailed customer feedback, BEH technology was selected as a second generation HPLC material for use in XBridge columns.
The key goals were to improve upon reversed phase performance by maximising efficiency, significantly increase pH stability and continue to improve the column reliability for method development work.
The most efficient route to a robust complex separation is the use of a wide pH range column, with the highest stability possible at both low and high pHs.
That column should ideally also be the most efficient possible, which is only achievable with the lowest tailing factors (especially for bases).
XBridge columns are said to answer all these needs with high pH stability levels that clearly separate it from today's available technology.
In accelerated high pH tests, the XBridge column lifetime reportedly exceeded that of a range of high performance commercially available columns by an order of magnitude (1000%).
This step function level of performance brings new buffer choices which have proven problematic in the past to the method developer.
XBridge columns are available in three particle sizes (2.5um, 3.5um and 5um) each with four chemistries (C18, C8, shield RP18, phenyl) in approximately 350 column dimension choices.
• Waters: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
