Product category:
Proteomics
News Release from: Wyatt Technology | Subject: Calypso
Edited by the Laboratorytalk Editorial
Team on 27 September 2007
Automated protein association and
aggregation
Calypso enables the rapid, quantitative, nondestructive characterisation of protein-protein interactions in native solution using composition-gradient multi-angle light scattering (CG-Mals)
Wyatt Technology announces the launch of its new Calypso automated protein association and aggregation system Interactions between proteins are at the heart of many biological functions and are of great importance for every process in a living cell
This article was originally published on Laboratorytalk on 9 Oct 2007 at 8.00am (UK)
Related stories
Calypso reduces prep time and noise levels
Application note details how scientists were able to more rapidly determine Mw and A2 with continuous flow to detectors, by accessorising Calypso automated protein association and aggregation system
Brochure describes new Wyatt Calypso system
The brochure, which is available free of charge, is entitled, 'Wyatt Calypso: Built-in automation leading to simple, reliable and repeatable measurements', and is available in PDF format only
Information about how proteins such as enzymes and inhibitors interact, improves our understanding of diseases and can point to new therapeutic approaches.
Characterisation of protein aggregation is critical to the pharmaceutical and biotechnology industries, where it is particularly important that the protein is in the solution phase in specific formulation buffers.
There are numerous methods for detecting protein interactions and aggregation kinetics, each varying in sensitivity and specificity.
CG-Mals is described as an ideal means for measuring the proteins in their native solution.
Protein samples need not be tagged, immobilised on a surface, or otherwise modified from their natural state.
It is a sensitive and precise method for the measurement of complexes and aggregates through the absolute determination of molar mass and can non-destructively characterise both weak and strong interactions.
Simple or complex association stoichiometries may be analyzed, providing equilibrium dissociation constants from picomolars to millimolars, as well as self- and cross- viral coefficients and aggregation or association kinetics.
The Wyatt Calypso system consists of hardware and dedicated software for automating CG-Mals measurements as well as Zimm plots and DN/DC determinations.
Computer-controlled syringe pumps automate the sample mixing, dilution and delivery, while intuitive software controls the experiment, acquires data and analyzes the results.
The system can also be used to facilitate the study of the composition dependence of any measurable solution property (spectroscopic, hydrodynamic, calorimetric).
• Wyatt Technology: contact details and other news
• Email this article to a colleague
• Register for the free Laboratorytalk email newsletter
• Laboratorytalk Home Page
Search the Pro-Talk network of sites
