Software for clinical DNA sequence analysis
SoftGenetics
Mutation Surveyor software
SoftGenetics' latest version of its Mutation Surveyor software uses enhanced technology to bring DNA variant calling from capillary electrophoresis sequences to a higher level by using many 'seeds'.
According to the company, primary advancements include two major algorithm improvements.
These are a reduction in false positive mutation calls in regions of poor dye mobility and a utilisation of 'seed' sequences within each trace to complete the alignment more quickly and effectively, which was originally developed for second-generation sequence alignment in SoftGenetics' NextGENe software.
Previously, Mutation Surveyor determined an initial alignment of a trace to the reference, then extended the alignment from this location.
By switching to using many 'seeds' the alignment can be completed more quickly.
Basecalls are used at the migration time of each peak to fine tune the alignment.
Mutation Surveyor has been validated for use in clinical sequence analysis by the NGRL, UK, and UPMC, Pittsburg PA, and is compatible with the output files of all major capillary electrophoresis systems including ABI Prism, Beckman Coulter CEQ and MegaBACE platforms.
More stories
GA workbench for next generation sequencing data
SoftGenetics has launched its web-based Geneticist Assistant NGS interpretative workbench.
Chimerism analysis software gains MCC test module
Softgenetics has added an automated test module for Maternal Cell Contamination (MCC) to Chimermarker, its automated Chimerism analysis and monitoring software.
Softgenetics upgrades Mutation Surveyor software
Softgenetics has released the latest version of its Mutation Surveyor software, which is designed for the analysis of DNA variants from Sanger sequence reads.
Software analyses data from sequencing platforms
Softgenetics has launched the newest version of its Nextgene software for the analysis of data from the Roche, Illumina, ION PGM and Applied Biosystems SOLID System sequencing platforms.
Analysis Corner for capillary and NGS analysis
Softgenetics has introduced the Analysis Corner, a knowledge base comprised of more than 20 freely accessible technical webinars dedicated to capillary and next-generation sequencing (NGS) analysis.
