E. coli cells exhibit high electroporation efficiencies

CloneCatcher Gold cells claim to meet the needs of scientists that are seeking to find rare clones or are building complex or metagenomic libraries.

Transferring exogenous DNA into E. coli is a standard laboratory method for cloning genes and constructing cDNA, genomic and epitope libraries.

However, the limiting factor in many library-screening efforts or multiple fragment ligations is the efficiency by which DNA can be introduced into E. coli.

Electroporation is one method to efficiently introduce DNA into E. coli.

CloneCatcher Gold cells exhibit extremely high electroporation efficiencies that approach the theoretical maximum of 3.4 x 10^11 cfu/µg pUC19 DNA.

The product requires less topoisomerase-loaded vector and therefore can reduce overall operating costs.

Supplied in single-use tubes - CloneCatcher Gold can also improve productivity as the whole protocol for use takes 10 minutes.