2D+ Cell Culture platform enables high content analysis

The company’s 2D+ Cell Culture Platform enables two new high content analysis (HCA) approaches: quantitative density mapping of trafficking compartments and standardised mitochondrial analysis.

The Cytoo Cell Culture Platform, which was launched in July 2012, is based on the use of adhesive micropatterns in microplate wells to guide the cell architecture and behavior in culture.

By defining the 2D topology of cell adhesion, the 2D+ Technology enables the fine control of the spreading and 3D shape of cultured cells in single- or multi-cellular configurations resulting in control of cell contractility, cell polarity, organelle positioning, or cell division axis.

This opens new perspectives for more physiologically relevant cell-based assays compared to classical 2D culture where cells spread and move in an uncontrolled manner.

The researchers of Bruno Goud’s team at the Institut Curie have presented novel data showing the use of the 2D+ Cell Culture Platform to identify proteins that sustain steady-state organisation of trafficking compartments by high-throughput density mapping in several compound and siRNA high content screens.

Following the method developed previously by the team, the hits were selected based on the P-value calculated from the difference between the density map of a pooled control and the density map of each treated condition in the screen.

The data from only 30 cells per condition was sufficient to get statistically significant results. In comparison, “state-of-the-art” HCA using unrestricted cells with considerable cell-to-cell variation requires hundreds or thousands of cells in order to get significant results.