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Andor confocal microscope used for live cell study

Andor Technology

Andor Revolution XD confocal microscope

A research team led by Prof Manojkumar Puthenveedu at Carnegie Mellon University is utilising the Andor Revolution XD confocal microscope for live cell studies.

Puthenveedu studies the mechanisms by which membrane trafficking controls and co-ordinates the complex signalling pathways in the brain.

Despite the fact that almost all diseases can be traced to a defect in how cells respond to signals, little is known about how signalling pathways are maintained in normal cells and the changes that occur in abnormal conditions.

Puthenveedu and his international team of researchers have used the Andor Revolution XD confocal microscope for live cell studies to elucidate how signalling receptors are recycled to the cell membrane.

The discovery of the mechanism by which signalling receptors travel back to the surface of the cell after activation and internalisation opens up a new class of therapeutic targets.

The team used live cell confocal fluorescence microscopy to label and image beta-2 adrenergic receptor (b2AR), the receptor for adrenaline and noradrenaline and one of a group of G protein-coupled receptors (GPCRs) pivotal to the regulation of heart and lung function, mood, cognition and memory, digestion, and the inflammatory response.

After internalisation, they found that b2AR was recycled via unique domains on the endosome, which they termed Actin-Stabilised Sequence-dependent Recycling Tubule (ASSERT) domains.

Rapid turnover of endosomal actin was confirmed by fluorescence recovery after photobleaching (FRAP).

The ASSERT domains trap and slow down the release of receptors to provide a slower pathway compared with the faster bulk recycling pathway and could potentially be targeted by pharmaceutical agents to control diseases resulting from abnormal cell signalling.

Mark Browne, director of systems at Andor, said: 'The high optical efficiency of the Revolution XD spinning disk system allows the use of reduced laser power and therefore lowers the risk of phototoxicity to the specimen during long-term experiments, while Andor's IQ software ensures precise synchronisation of illumination, detection and scanning for unparalleled image quality and acquisition rates.'

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