Evolve camera uses photoelectron to measure images

Previously, scientific-grade CCD cameras provided data in arbitrary imaging units, making it time consuming and challenging to reproduce study results.

Evolve's Quant-View feature provides a repeatable methodology to gather and interpret data by reading out pixel values in photoelectrons.

By using Evolve in Quant-View mode, investigators can ensure that their experimental data are well controlled, consistent and reproducible within and across laboratories.

Deepak Sharma, Photometrics' camera product manager, said: 'Scientific research is often hindered by conflicting data and inconsistent data measurement, resulting in delays, loss of funding and disputable study outcomes.' With the Evolve camera, researchers can now attain a pure measurement of a scientific image, which is important considering the growing popularity of ultra-quantitative techniques such as photo-activated localisation microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), where certainty of measurement is said to be essential.

Other features of Evolve include: Rapid-Cal, which provides an accurate, simple and fast EM calibration technique; background event reduction technology (BERT), which enables researchers to correct spurious event data; Top-Lock and Black-Lock, which are complementary intensity filtering tools that allow researchers to narrow visualisation to the intensity range of the image features under study; and a deep-cooled platform with a low dark current, a low read noise and a vacuum guarantee.

The camera is suitable for life-science applications including super-resolution fluorescence microscopy, such as PALM and STORM, as well as low-light applications such as spinning disk confocal microscopy, total internal reflection fluorescence (TIRF) microscopy, cell trafficking studies, live-cell fluorescent protein imaging and single-molecule fluorescence (SMF).