Evolve camera uses photoelectron to measure images
Evolve EMCCD camera
Photometrics has introduced the Evolve EMCCD camera, which makes experimental imaging data quantifiable and reproducible by using the photoelectron to scientifically measure an image.
Previously, scientific-grade CCD cameras provided data in arbitrary imaging units, making it time consuming and challenging to reproduce study results.
Evolve's Quant-View feature provides a repeatable methodology to gather and interpret data by reading out pixel values in photoelectrons.
By using Evolve in Quant-View mode, investigators can ensure that their experimental data are well controlled, consistent and reproducible within and across laboratories.
Deepak Sharma, Photometrics' camera product manager, said: 'Scientific research is often hindered by conflicting data and inconsistent data measurement, resulting in delays, loss of funding and disputable study outcomes.' With the Evolve camera, researchers can now attain a pure measurement of a scientific image, which is important considering the growing popularity of ultra-quantitative techniques such as photo-activated localisation microscopy (PALM) and stochastic optical reconstruction microscopy (STORM), where certainty of measurement is said to be essential.
Other features of Evolve include: Rapid-Cal, which provides an accurate, simple and fast EM calibration technique; background event reduction technology (BERT), which enables researchers to correct spurious event data; Top-Lock and Black-Lock, which are complementary intensity filtering tools that allow researchers to narrow visualisation to the intensity range of the image features under study; and a deep-cooled platform with a low dark current, a low read noise and a vacuum guarantee.
The camera is suitable for life-science applications including super-resolution fluorescence microscopy, such as PALM and STORM, as well as low-light applications such as spinning disk confocal microscopy, total internal reflection fluorescence (TIRF) microscopy, cell trafficking studies, live-cell fluorescent protein imaging and single-molecule fluorescence (SMF).
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