EtG analysis sample-preparation application note

Ethyl glucuronide (EtG) can be detected at very low levels and, being a metabolite, it is useful in distinguishing between ingested ethanol and that which is absorbed through the skin from ethanol-containing products such as alcohol-based hand washes.

Rapid and reliable methods with robust extraction protocols are essential for analysis and quantitation of EtG from various matrices, using liquid chromatography-mass spectrometry (LC-MS).

Advances in the manufacture of polymeric stationary phases and the recent release of EVOLUTE AX, a mixed-mode ion-exchange product, have allowed for the development of a more robust, cost-effective procedure.

The protocol was developed on 96-well plates for high-throughput, with volumes outlined for scale-up to cartridges in manual or automated systems.

EVOLUTE AX sample preparation products are available in a range of standard configurations and two different particle sizes (30um and 50um) to suit a wide variety of applications.

The mixed-mode sorbent chemistry and optimised pore size minimises retention of high molecular weight matrix components present in the biological fluid samples.

Interferences including proteins, salts, non-ionisable molecules and phospholipids are removed during the interference elution step, thereby ensuring extremely clean extracts.

Biotage sample-preparation products are supported by a wealth of knowledge and experience in method development and trouble shooting.