Characterising biomolecules and nanoparticulates

Visitors to the Viscotek exhibit at Analytica will be able to see and discuss, with technical specialists, a new generation of the model 802 dynamic light scattering (DLS) instrument - the 802DAT.

Traditionally, laboratories faced with analysing higher concentration samples, such as protein crystallography scientists, protein NMR scientists, emulsions or nanoparticle researchers, have found traditional light scattering instruments limited both in terms of sensitivity and resolution.

To address these deficiencies Viscotek developed as an option for its new generation system - dual attenuation technology.

Dual attenuation technology (DAT) controls the level of light entering the sample as well as controlling scattered light going to the detector.

In this way, for the first time, researchers are able to work at the optimum conditions for the laser and detector irrespective of sample type.

Designed for laboratories that need a complete understanding of how their natural or synthetic macromolecule behaves in solution the model 802DAT sets a new standard for superior sensitivity, resolution, accuracy and sampling versatility compared to any other light scattering instrument currently available.

Incorporating Viscotek's patented single mode fibre (SMF) optical detection, dual attention technology (DAT) and independent temperature control of microplate and sample the HorizonDLS High-Throughput DLS plate reader sets a benchmark for automated biomolecular characterisation.

The HorizonDLS delivers the same unmatched sensitivity on ultra low sample volumes as the model 802 DLS, but automatically, from the entire plate or selected wells, with no user interaction required.

After manual or robotic placement of a prepared microplate into the temperature controlled instrument, Omnisize software then automatically makes the measurements according to the user programmed acquisition sequence and the results are displayed in seconds.

A complete size distribution of an entire microplate can be automatically accomplished in just minutes.

The HorizonDLS uniquely delivers both productivity and performance to a broad range of applications including screening of aggregates prior to protein crystallisation, monitoring size and aggregation as a function of varying solution conditions, buffer optimisation and characterisation and screening biotherapeutics for self-association.

For polymer, biopolymer and protein scientists visiting the show Viscotek will display its triple detector array (TDA) and popular GPCmax systems.

The Viscotek TDA sets the standard for GPC/SEC technology and is the preferred method for characterising natural and synthetic macromolecules.

The TDA employs a concentration detector, viscometer and light scattering detector acting in concert, with each detector providing complimentary but different information.