Cambridge BioScience has introduced NucView Caspase Substrate to its wide range of innovative fluorescent reagents for life science research
The substrate is the first to use Biotium's CellView enzyme detection technology, designed for detecting intracellular or extracellular enzyme activity of live cells.
CellView caspase substrates are bi-functional; able to detect the presence of a caspase and then fluorescently stain a specific cellular component - in this case, cell nuclei.
CellView caspase substrates consist of a fluorogenic functional dye and a covalently-linked caspase substrate moiety that renders the functional dye non-functional.
Cleavage of the substrate by a caspase releases the fluorogenic functional dye which then becomes highly fluorescent upon binding to a cellular component.
This unique property of CellView caspase substrates makes them an ideal tool for studying apoptopic cells by fluorescence microscopy or flow cytometry.
