Cisbio Bioassays has announced the launch of the HTRF-based Cellul'erk assay for phosphorylated-ERK1/2 investigation.
The kit, designed for the exploration of G-protein Coupled Receptor (GPCR) signaling pathways, is the latest addition to Cisbio's HTRF-based platform of functional tools for GPCR and cell-surface receptor investigation.
Cellul'erk is a cell-based assay that directly detects endogeneous phosphorylated ERK1/2.
This efficient, functional readout for exploring GPCR signalling pathways in a high-throughput mode targets ERK (extracellular signal-regulated kinase), whose phosphorylation can be modulated upon GPCR activation through G-protein or B-Arrestin.
Its straightforward assay protocol and antibody-based detection provides a rapid alternative to Western blot or Elisa that is sensitive enough for endogeneous and over-expressed receptors, and can be miniaturised to 1536-well formats.
