Thermo Fisher Scientific offers a wide range of Macherey Nagel products for nucleic acid purification, including the Nucleospin Robot-96 kit for the isolation of plasmid DNA in a 96-well plate format.
The Molecular Biology Core Facility at the Paterson Institute for Cancer Research (a Cancer Research UK-funded division of the University of Manchester) has used the Macherey Nagel Nucleospin Robot-96 Plasmid kit for the last six months to provide purified plasmid DNA for downstream applications such as sequencing, cloning and transfections.
'We provide a range of core services for research groups on site, including plasmid preparation,' said Stuart Pepper, head of the Molecular Biology Core Facility.
'Our main reasons for switching to the Macherey Nagel Nucleospin product were quality and yield.
'Plasmid preparation is a very routine task for us, generally used for routine checking of clones during molecular biology projects.
'Using Nucleospin chemistry has given us a reliable product with improved quality of plasmid DNA extracts.
'The preparations we obtain are now of sufficient quality for transfections and the yield is also very good.
'When we decided to trial the product, the chemistry was up and running in a single day, with minimal interruption to our work.
'We ran the chemistry for a week in an automated method using our existing robotic system, extending the trial period to one month when we saw the benefits in terms of DNA quality.
'At the end of the trial, we opted to start using the Macherey Nagel Nucleospin product routinely,' he added.
The Nucleospin Robot-96 Plasmid kit is said to be ideal for automated processes, providing high-quality plasmid DNA preparations that are ready to use for downstream applications.
Macherey Nagel Nucleospin products are available to UK scientists through Thermo Fisher Scientific.
