Evrogen is offering its normalisation of genomic DNA service for further next-generation sequencing.
Whole-genome sequencing (WGS) is a powerful approach for obtaining reference sequence information for multiple organisms.
In the case of large genomes, the presence of repetitive sequences, which constitute a significant proportion of total genome size, makes WGS impractical.
Elimination of the repetitive DNAs facilitates sequencing and analysis of large genomes.
Evrogen duplex-specific nuclease (DSN)-normalisation technology is a efficient and proved approach to equalise transcript abundance in cDNA populations enriched with full-length sequences.
DSN-normalisation applied for genomic DNA is effective against abundant repetitive elements with high sequence identity and satellite DNAs, while retaining highly divergent repeats and coding regions at baseline levels.
In other words, DSN-normalisation can be used to eliminate evolutionarily young repetitive sequences from genomic DNA before sequencing, and should prove invaluable in studies of large eukaryotic genomes, such as those of higher plants.
