The Cellreporter system from Genetix now supports both cell- and bead-based assay formats to support research projects and provide data that is essential in drug discovery and development workflows.
Users can screen cell-by-cell or bead-by-bead within a test population in order to produce accurate, quantitative data for a range of applications.
Typically used to quantify the amount of protein secreted by a cell line, bead-based assays are said to be key to applications such as hybridoma screening, assessing production of antibodies, cytokines or hormone secretion.
The homogeneous format of the bead-based assays used with Cellreporter is claimed to simplify the process compared with conventional approaches, as it involves only a single incubation step and eliminates the need for multiple wash steps.
Cell-based arrays, including cell cycle analysis, cytotoxicity studies and monitoring protein translocation, are used as biological models of physiological situations, for example, when investigating the effect of drug candidates on a cancer cell model.
The Cellreporter system enables scientists to increase throughput without comprising image or data quality, according to Genetix.
In addition, the accuracy of the quantitative data can be improved compared with conventional approaches.
Flexible image analysis software identifies and analyses each object, such as the cell nucleus or bead.
Users can optimise and standardise image interpretation for each assay and check data quality, for example, to remove fluorescent debris that may compromise results.
