Sigma Life Science has released its pre-designed CompoZr Knockout Zinc Finger Nucleases (ZFNs) technology to affordably knockout any gene in the human genome.
The offer can generate permanent and heritable gene knockouts in human cell lines within weeks, and has been priced to fit within the budget of a typical laboratory.
Sigma Life Science said few laboratories currently create gene knockouts in human cell lines because the inefficiencies of the natural cellular homologous recombination system and the investment of time and labour required to screen the resulting cells.
In contrast, a wealth of peer-reviewed literature has shown that CompoZr ZFNs efficiently generate human cell lines with precise and stable gene knockouts in weeks.
In this offering, Sigma has designed CompoZr ZFNs that will produce a knockout in each gene by inducing a double strand break at a defined site within the gene's first three exons.
This double strand break stimulates the cell's natural DNA repair pathways, resulting in a permanent, site-specific deletion or mutation that disrupts the gene's function.
Robust and on-target gene knockout using CompoZr Knockout ZFNs is said to be guaranteed by functional validation prior to shipment and enhancements to the DNA-recognition and cleavage ZFN subunits.
All CompoZr Knockout ZFNs can be ordered directly through the Your Favorite Gene powered by Ingenuity web portal.
More sophisticated genetic modifications, such as single-nucleotide substitutions, can be generated through Sigma Life Science's CompoZr Custom ZFN Service.
Also, the CompoZr Targeted Integration Kit allows rapid integration and expression of a gene of interest at the well-characterised adeno-associated virus integration site.
