Sigma Life Science, part of Sigma-Aldrich, has launched the mRosa26, a CompoZr targeted integration kit that can integrate transgenes into the mouse genome at the Rosa26 safe harbour locus.
This kit follows the introduction of the AAVS1 CompoZr targeted integration kit for transgene integration in the human genome.
Traditional transgenic approaches rely on the random integration of a transgene.
This approach results in multiple random integration events at off-target insertion sites and often requires the use of antibiotic selection strategies.
In addition, the integration event can involve multiple copies of the transgene.
Using the CompoZr zinc finger nuclease (ZFN) technology, a single copy of a transgene can be targeted and inserted in the Rosa26 locus, which is a well-defined safe harbour site in the mouse genome.
Sigma's CompoZr targeted integration kits use ZFNs for precise and heritable gene knock-in at the target locus (Rosa26 or AAVS1).
Both human and mouse kits are supplied with the necessary ZFNs and donor plasmid to successfully perform transgene integration by ZFN-assisted homologous recombination, reducing the timescale and complexity of engineering transgenic mouse and human cell lines.
