High speed microbiological detection technique is based on solid-phase sandwich hybridation with luminescent detection to identify pathogens in under 24 hours
In the field of food diagnosis, manufacturers want accelerated analyses.
Europrobe says it has perfected a series of rapid tests that detect the presence of pathogen agents.
The technology is said to be easy to use, rapid and more sensitive than existing tests, and gives results (from RNA bacterial extraction to the analysis, including the enrichment phase) in 24 hours maximum. Europrobe is the only company to offer a compact, fully automated chain for bacterial DNA/RNA hybridation in in the solid phase. Europrobe has developed the Lumiprobe 24, ultra-fast diagnostic technology in 24 hours.
Its analyser is completely automated and very easy to use.
It detects listerisp and Listeria monocytogenes, salmonella sp.
The company has French approval to detect salmonella sp in human and animal foods.
The technique is based on solid-phase sandwich hybridation with luminescent detection.
The technique can detect targeted bacteria in less 24 hours, most of wich time is taken up by culture of the original sample in an enrichment broth common for broth salmonella and listeria.The test itself requires four steps and takes two hours.The procedure begins with sampling of the enrichment broth and lysis of bacteria in it.
The lysed sample is then placed in a tube (for small-scale testing) or in a well of a microtiter plate.
The tube or well has been previously coated with a DNA oligonucleotide complementary to a region on the ribosomal RNA of the target bacteria.
Use of ribosomal RNA, which is present in thousands of copies per cell, greatly increases the sensitivity of the assay, eliminating the need to use PCR methods to amplify RNA.
The RNA bound by this capture is then further hybridised with another DNA oligonucleotide, a 'reading probe' that is florescein-labelled.
The test tube or plate is read using a standard chemiluminometer.
