Superparamagnetic beads promise a quicker and easier column-free method to prepare for mass spectrum analysis using reversed phase chromatography (RPC) or weak cation exchange (WCX)
Dynal has announced the release of a new product in its proteomic preparation range - Dynabeads RPC 18.
Together with the recently launched Dynabeads WCX and Dynabeads RPC protein, these products are said to offer an excellent alternative to chromatography columns, spin columns and pipette tips sample preparation technologies.
Based on reversed phase chromatography (RPC) or weak cation exchange (WCX), these beads can fractionate mixed protein and peptide samples quickly and easily for downstream analysis such as mass spectrometry.
Dynabeads RPC 18 are designed with an optimised surface for binding of intricate peptide mixtures with possible downstream fractionation.
The magnetic separation methodology is highly reproducible, flexible and scalable, and can be automated on a wide variety of liquid handling platforms.
According to international product manager Hege Skjellerudsveen, the magnetic bead chromatography product range will help reduce sample complexity.
"These three Dynabeads products can be used to concentrate, prepare and desalt complex protein and peptide mixtures.
"Both top-down and bottom-up approaches can be employed.
"Acting as a controllable solid phase support, the beads respond to a magnetic field allowing bound material to be rapidly and precisely separated from salts and other contaminants.
"Proteins or peptides are then eluted off or the sample is fractionated by eluting step-wise.
"Since the beads use different properties they can be used to isolate different fractions from the same sample.
"This means that they provide a powerful and adaptable preparation protocol for downstream mass spectrum analysis, HPLC and 1D/2D gel electrophoresis, yielding highly reproducible results".
All Dynabeads are engineered to provide uniform spheres with even magnetic properties and maximum surface area.
They remain dispersed in solution but very quickly move in response to a magnetic field, with no magnetic remenance (superparamagnetic).
The different beads available have been designed to accommodate many different surface chemistries and have been developed in a number of different sizes to suit different proteomic, genomic and in vitro diagnostic applications.
Dynal is a pioneer of biomagnetic separation technology and holds the original patents for superparamagnetic beads.
Today, the Dynabeads portfolio supports cell separation for basic and clinical research, genomics, transcriptomics, proteomics, diagnostics and more.
In addition, Dynal offers tailor-made products in collaboration with large volume users.
Dynabeads are superparamagnetic: that is, they exhibit magnetic properties only when placed within a magnetic field and show no residual magnetism when removed from this field.
The polymer shell of the beads protects targets from toxic exposure to iron.
The true uniformity (CV<3%) of size, shape and surface area provides optimal accessibility and reaction kinetics, allowing for rapid and efficient binding.
The true spherical shape and defined surface chemistry minimise chemical agglutination and non-specific binding.
The specific characteristics of the many available bead types facilitate magnetic separation of a wide variety of targets.
Unique batch-to-batch reproducibility (typically within 5%) secures the reproducibility and quality of results, says Dynal.
Separation is gentle and no column or centrifugations are necessary.
