Improved cryopreservation of human hepatocytes
A report from researchers at the Karolinska Institute details an improved protocol for cryopreservation of primary human hepatocyte.
In the article the authors compare cryopreservation of liver hepatocytes using STEM-CELLBANKER with the frequently used DMSO-University of Wisconsin (DMSO-UW) medium protocol.
Both protocols yielded hepatocytes with good P450 function, however, the STEM-CELLBANKER protocol gave a higher viability than the DMSO-UW cryoprotectant. The researchers concluded that STEM-CELLBANKER beneficially is also xeno-free and will be applicable to cryopreservation of clinical-grade primary human hepatocytes.
STEM-CELLBANKER from AMSBIO is a chemically defined freezing medium with optimized formulation for stem cells and iPS cells storage as well as other valuable cells.
Supplied ready-to-use with a simple usage protocol, STEM-CELLBANKER is completely free of serum and animal derived components, and contains only European or US Pharmacopoeia graded ingredients.
Part of the CELLBANKER® series of cell freezing media, STEM-CELLBANKER® significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage.
Every batch of STEM-CELLBANKER® cryopreservation medium is performance tested on Jurkat and SK-007 cells. Additional standard evaluations for endotoxins, pH, osmolarity and Mycoplasma contaminants are undertaken to ensure GMP equivalent quality.
The service will now include blood products from human donors with auto-immune and neurological conditions.
Entitled ‘Short Guide to 3D Cell Culture’, the booklet considers a range of procedures for cell culture.
Stem Cellbanker has proven to be an effective freezing medium for stem cells and iPS cells storage.
AMSBIO has introduced highly purified Chondroitinase ABC for neural regeneration research.
AMSBIO has announced a range of genomic DNA and cDNA products for immediate identification of genes.