NEXTflex kit improves NGS methylome analysis
The first ever kit to simplify library preparation of bisulfite treated DNA has been released by Bioo Scientific.
Bisulfite-seq is an important method for studying DNA methylation and epigenetic gene regulation as it allows for an unbiased genome-wide analysis of DNA methylation generating a high-accuracy methylome.
NEXTflex Bisulfite-Seq Kit is designed to work with reduced representation or total bisulfite-converted DNA libraries and is compatible with single, paired-end and multiplexed DNA libraries on Illumina MiSeq, GAIIx and HiSeq platforms.
It includes Bioo Scientific’s proprietary “Enhanced Adapter Ligation Technology” which offers improved ligation efficiency than other commercially available solutions resulting in library preps with a larger number of unique sequencing reads.
This kit also uses a completely gel-free protocol making the workflow compatible with liquid handler automation. For multiplexing, NEXTflex™ Bisulfite-Seq Barcodes can be purchased separately.
According to Dr. Masoud Toloue, Director of Genomic Research at Bioo Scientific, “Short of direct sequencing of methylated cytosines, Bisulfite-Seq is currently the most sensitive and accurate method of elucidating epigenetic marks.”
Bioo Scientific has also launched the NEXTflex Msp1 Restriction Enzyme, which unlike other commercially available Msp1 restriction enzymes, has been optimised for bisulfite sequencing.
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