PCR reagent offers accurate detection of SNP
Bio-Rad Laboratoriescontact supplier
Precision Melt Supermix
Bio-Rad Laboratories has introduced the Precision Melt Supermix, designed to deliver HRM performance for detection of single nucleotide polymorphisms (SNP) and CpG methylation for epigenetic studies.
The PCR reagent is compatible with all HRM-capable thermal cyclers.
Traditional genotyping methods, such as denaturing high-pressure liquid chromatography (dHPLC) and single-strand conformation polymorphism (SSCP), involve lengthy protocols and require multiple days for completion.
Probe-based genotyping can be expensive, especially for large numbers of SNPs.
HRM is designed to be a simple, rapid and inexpensive alternative for studying genetic variation.
Precision Melt Supermix was optimised specifically for accurate, robust and reproducible HRM discrimination.
By employing the saturating Evagreen dye, which is required for detection of class III/IV SNPs, the supermix discriminates all four SNP classes across a broad range of amplicons.
This allows for any genomic region to be studied, regardless of amplicon length and GC content.
The supermix is also fully compatible with bisulphite-converted DNA and allows for accurate detection of CpG methylation status.
Furthermore, Precision Melt Supermix's room-temperature stability allows researchers to screen thousands of samples using Bio-Rad's CFX96 Touch or CFX384 Touch real-time PCR systems with the CFX automation system for high-throughput HRM analysis.
Rapid cell lysis RT-qPCR kits download
Bio-Rad Laboratories has launched rapid cell lysis kits that can obtain reverse transcription quantitative PCR (RT-qPCR) data directly from cultured cells without a separate RNA purification step.
Bio-Rad Laboratories has launched the SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, a proven solution for obtaining reproducible, optimal-quality qPCR data from the most challenging samples.
SureBeads for immunoprecipitation download
Bio-Rad Laboratories has launched the SureBeads Magnetic Bead System, designed to offer a fast and easy alternative to agarose beads for immunoprecipitation (IP).
Bio-Rad's latest supermix assays download
Bio-Rad Laboratories has launched the SsoAdvanced PreAmp Supermix and PrimePCR PreAmp Assays.
Bio-Rad Laboratories has launched CytoTrack Cell Proliferation Assays, designed to provide unmatched flexibility when designing a multicolour flow cytometry experiment.